GRP94 Protein (full length) (rho-1D4 tag)
-
- 抗原 See all GRP94 (HSP90B1) 蛋白
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
- 蛋白类型
- Recombinant
- 产品特性
- full length
-
宿主
- CHO cells
-
资源
- Insect Cells
- 标记
- This GRP94 protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
-
MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDEAAK EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE SEKTKENREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR VKEDEDDKTV LDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID PEAQVEEEPE EEPEDTTEDT EQDEEEEVDA GTEEEEEEEQ ETAKESTAEK DEL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Endoplasmin (Heat shock protein 90 beta family member 1) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product HSP90B1 蛋白
-
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 1-315) protein (GST tag)
HSP90B1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 282-409) protein (His-IF2DI Tag)HSP90B1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 23-315) protein (GST tag)HSP90B1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 22-803) protein (His tag)HSP90B1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 22-803) protein (His tag)Crystallography grade HSP90B1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 22-802) protein (His tag)Crystallography grade HSP90B1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
- 别名
- Endoplasmin (Heat shock protein 90 beta family member 1) (HSP90B1 产品)
- 别名
- ecgp Protein, gp96 Protein, tra1 Protein, grp94 Protein, DDBDRAFT_0206450 Protein, DDBDRAFT_0215015 Protein, DDB_0206450 Protein, DDB_0215015 Protein, TRA1 Protein, HSP90B1 Protein, ERp99 Protein, GRP94 Protein, TA-3 Protein, Targ2 Protein, Tra-1 Protein, Tra1 Protein, endoplasmin Protein, GP96 Protein, fb61d09 Protein, tra-1 Protein, wu:fb61d09 Protein, wu:fq25g01 Protein, Grp94 Protein, ECGP Protein, Gp96 Protein, ppk98 Protein, hsp108 Protein, heat shock protein 90kDa beta (Grp94), member 1 Protein, heat shock protein Hsp90 family protein Protein, 94 kD glucose-regulated protein Protein, heat shock protein 90 beta family member 1 Protein, heat shock protein 90, beta (Grp94), member 1 Protein, heat shock protein 90, beta (grp94), member 1 Protein, heat shock protein 90kDa beta family member 1 L homeolog Protein, hsp90b1 Protein, grp94 Protein, HSP90B1 Protein, Hsp90b1 Protein, hsp90b1.L Protein
- UniProt
- G3HQM6
- 途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
-
