EEF2 Protein (full length) (rho-1D4 tag)
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- 抗原 See all EEF2 蛋白
- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This EEF2 protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MVNFTVDQIR AIMDKEANIR NMSVIAHVDH GKSTLTDSLV CKAGIIASAR AGETRFTDTR KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGSGFLI NLIDSPGHVD FSSEVTAALR VTDGALVVVD CVSGVCVQTE TVLRQAIAER IKPVLMMNKM DRALLELQLE PEELYQTFQR IVENVNVIIS TYGEGESGPM GNIMIDPVLG TVGFGSGLHG WAFTLKQFAE MYVAKFAAKG EGQLGPAERA KKVEDMMKKL WGDRYFDPAN GKFSKSANSP DGKKLPRTFC QLILDPIFKV FDPIMNFRKE ETAKLIEKLD IKLDSEDKDK EGKPLLKAVM RRWLPAGDAL LQMITIHLPS PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK MVPTSDKGRF YAFGRVFSGV VSTGLKVRIM GPNYTPGKKE DLYLKPIQRT ILMMGRYVEP IEDVPCGNIV GLVGVDQFLV KTGTITTFEH AHNMRVMKFS VSPVVRVAVE AKNPADLPKL VEGLKRLAKS DPMVQCIIEE SGEHIIAGAG ELHLEICLKD LEEDHACIPI KKSDPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKA RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR GSQVVAETRK RKGLKEGIPA LDNFLDKL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Elongation factor 2 (EF-2) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product EEF2 蛋白
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Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 1-858) protein (GST tag)
EEF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 2-858) protein (His tag)Crystallography grade EEF2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 2-858) protein (His tag)Crystallography grade EEF2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 574-858) protein (His tag)EEF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 2-858) protein (His tag)EEF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Eukaryotic Translation Elongation Factor 2 (EEF2) protein (Myc-DYKDDDDK Tag)EEF2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
- 别名
- Elongation factor 2 (EF-2) (EEF2 产品)
- 别名
- EEF-2 Protein, EF-2 Protein, EF2 Protein, Ef-2 Protein, eef2 Protein, MGC76191 Protein, MGC79628 Protein, EEF2 Protein, eef2l Protein, fe49h02 Protein, wu:fe49h02 Protein, zgc:63584 Protein, ef2 Protein, zef2 Protein, CG2238 Protein, Dmel\CG2238 Protein, EF-2b Protein, EF2B Protein, EF2b Protein, Ef2 Protein, Ef2B Protein, Ef2b Protein, anon-EST:Liang-1.44 Protein, chr2L:21668915..21669164 Protein, clone 1.44 Protein, eEF2 Protein, eF2 Protein, ef2b Protein, eukaryotic translation elongation factor 2 Protein, eukaryotic translation elongation factor 2, gene 1 Protein, eukaryotic translation elongation factor 2b Protein, Elongation factor 2 Protein, EEF2 Protein, Eef2 Protein, eef2.1 Protein, POSPLDRAFT_118836 Protein, eef2b Protein, eef-2 Protein, eef2 Protein, EF2 Protein
- 背景
- Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. {ECO:0000269|PubMed:7559470}.
- UniProt
- P09445
- 途径
- AMPK Signaling
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