CD3D & CD3E (AA 23-126) (Active) protein (His tag,AVI tag,Biotin)
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- 抗原
- CD3D & CD3E
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-126
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- His tag,AVI tag,Biotin
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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CD3D & CD3E (AA 23-126) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-120) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-126) protein (Fc-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
CD3D & CD3E (AA 23-120) protein (His tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
CD3D & CD3E (AA 23-108) protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-126) (Active) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (Fc Tag,His tag,DYKDDDDK Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 应用备注
- MALS verified
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
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- 抗原
- CD3D & CD3E
- 别名
- CD3E & CD3D
- Sub Type
- Cocktail
- 背景
- T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
- 分子量
- 18.6 kDa (CD3E) and 13.4 kDa (CD3D)
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