SARS-CoV-2 NSP16, NSP10 protein (His tag,Twin-Strep tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: 大肠杆菌（E. Coli）
Recombinant
>90 % as determined by SDS-PAGE.
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- 抗原
- SARS-CoV-2 NSP16, NSP10
- 蛋白类型
- Recombinant
- 宿主
- SARS Coronavirus-2 (SARS-CoV-2)
- 资源
- 大肠杆菌（E. Coli）
- 标记
- His tag,Twin-Strep tag
- 原理
- SARS-CoV-2 (COVID-19) NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag
- 序列
- AA 1-298
- 产品特性
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SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag (NS0-C51W3) is expressed from E.coli cells. It contains AA Ser 1 - Asn 298 (NSP16) & Ala 1 - Gln 139 (NSP10) (Accession # YP_009725311.1 (NSP16) & YP_009725306.1 (NSP10). Predicted N-terminus: Met (NSP16) & Met (NSP10)
SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag is produced by co-expression of NSP16 and NSP10, has a calculated MW of 35.2 kDa (NSP16) and 17.4 kDa (NSP10). Subunit NSP16 is fused with a polyhistidine tag at the N-terminus and subunit NSP10 is fused with a Twin Strep tag at the N-terminus. - 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -80 °C
- 储存方法
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The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.
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- 抗原
- SARS-CoV-2 NSP16, NSP10
- 别名
- SARS-CoV-2 NSP16 & NSP10
- 物质类
- Viral Protein
- 背景
- NSP10, Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and 2'-O-methyltransferase (NSP16) activities. Therefore plays an essential role in viral mRNAs cap methylation. 2'-O-methyltransferase (NSP16) that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16, these interactions enhance nsp14 and nsp16 enzymatic activities.
- 分子量
- 35.3 kDa (NSP16) & 18.3 kDa (NSP10)
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