SARS-CoV-2 NSP3 Protein (His tag)
NSP3
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: 大肠杆菌（E. Coli）
Recombinant
>95 % as determined by SDS-PAGE.
SDS
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(1 reference)-
- 抗原 See all SARS-CoV-2 NSP3 (NSP3) 蛋白
- SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))
- 蛋白类型
- Recombinant
- 宿主
- SARS Coronavirus-2 (SARS-CoV-2)
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SARS-CoV-2 NSP3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 原理
- SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag
- 序列
- AA 746-1060
- 产品特性
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SARS-CoV-2 Papain-like Protease, His Tag is expressed from E.coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus. - 纯度
- >95 % as determined by SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product NSP3 蛋白
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SARS-CoV-2 Papain-Like Protease (NSP3) (AA 1564-1878) protein (His tag)
NSP3 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS-PAGE. SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 7.5
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
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This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt,
-70°C for 3 months under sterile conditions.
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: "Antibody Responses to SARS-CoV-2 Antigens in Humans and Animals." in: Vaccines, Vol. 8, Issue 4, (2020) (PubMed).
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: "Antibody Responses to SARS-CoV-2 Antigens in Humans and Animals." in: Vaccines, Vol. 8, Issue 4, (2020) (PubMed).
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- 抗原
- SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))
- Abstract
- NSP3 产品
- 别名
- pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein
- 物质类
- Viral Protein
- 背景
- New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.
- 分子量
- 37.6 kDa
- NCBI登录号
- YP_009725299
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