ZEBOV GP Protein (AA 33-501) (His tag)
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- 抗原 See all ZEBOV GP products
- ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 33-501
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宿主
- Ebola Virus
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资源
- HEK-293 Cells
- 标记
- This ZEBOV GP protein is labelled with His tag.
- 原理
- Recombinant Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) Envelope Glycoprotein 1 (GP1) protein produced in HEK293 cells. Protein contains a C-terminal 6x His-tag
- 特异性
- Ebola virus envelope glycoprotein is initially produced as a precursor which is then cleaved into two subunits, GP1 and GP2. This is a recombinant version of GP1, produced in HEK293 cells, and incorporating a C-terminal 6x His-tag with greater than 95% purity.
- 产品特性
- Ebola Virus Envelope Glycoprotein (GP1) (Zaire)
- 纯度
- >95 %
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- 说明
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This Ebola virus envelope glycoprotein (GP1) is derived from the GP1 sequence (Accession # AAQ55048.1), expressing Ile33 – Arg501. It is fused with a polyhistidine tag at the C-terminus and has a calculated MW of 51.6kDa. The predicted N-terminus is Ile 33. The protein is expressed in HEK293 cells. DTT-reduced protein migrates as 120-140kDa in SDS-PAGE.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C
- 储存方法
- 4°C
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- 抗原
- ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
- 别名
- Ebola Envelope Glycoprotein (GP1) (Zaire) (ZEBOV GP 产品)
- 物质类
- Viral Protein
- 背景
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Ebola virus envelope glycoprotein is initially produced as a precursor known as pre-GP, which is cleaved by furin into two subunits, GP1 and GP2, which remain associated through a disulfide linkage between Cys53 of GP1 and Cys609 of GP2. This heterodimer assembles into a 450-kDa trimer at the surface of nascent virions.
The virion-attached GP is critical in the EBOV life cycle, as it is solely responsible for attachment, fusion and entry of target cells. Moreover, GP is responsible for critical pathogenic differences among viral species. - UniProt
- AAQ55048.1
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