Quick Overview for GDNF 蛋白 (ABIN6700939)
抗原
See all GDNF 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-v10-0010
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Supplier
- Rockland
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原理
- Mouse Glial Derived Neurotrophic Factor Recombinant Protein
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纯化方法
- Glial Derived Neurotrophic Factor purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of C6 cells and is typically less than 1 μg/mL.
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Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein
GDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)GDNF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 1-211) protein (GST tag)GDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (Fc Tag)GDNF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glial Cell Line Derived Neurotrophic Factor (GDNF) proteinGDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Glial Cell Line Derived Neurotrophic Factor (GDNF) proteinGDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
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应用备注
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Application Note: Glial Derived Neurotrophic Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Glial Derived Neurotrophic Factor in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
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别名
- Gdnf
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背景
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Synonyms: ATF-1
Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a neurotrophic factor that is closely related to other neurotrophic factors, such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes RET and GFRα1-4, to promote dopamine uptake, survival and differentiation of neurons. Recombinant mouse GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains, with a total molecular weight of 30.2 kDa.
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UniProt
- P48540
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途径
- RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
抗原
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