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EGF 蛋白

Name: EGF 蛋白
SKU: ABIN6699794
Availability: OutOfStock

This Recombinant EGF protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.

(2 references)

产品编号 ABIN6699794

Supplier Product No.: 009-001-c26

发货至: 中国

Quick Overview for EGF 蛋白 (ABIN6699794)

抗原

See all EGF 蛋白

EGF (Epidermal Growth Factor (EGF))

蛋白类型

Recombinant

宿主

人

资源

大肠杆菌（E. Coli）

纯度

95,00%

Discover our top product EGF 蛋白

Supplier Product No.

009-001-c26

Supplier

Rockland

原理

EGF Human Recombinant Epidermal Growth Factor

纯化方法

Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.

内毒素水平

Measured by LAL is 0.5 EU/μg protein.

Biological Activity Comment

The ED50, calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells, is 30-40 pg/ml.
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor (EGF) (Active) protein
EGF 宿主: 人宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active

Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)
EGF 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)
EGF 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)
EGF 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Epidermal Growth Factor (EGF) (Active) protein
EGF 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active

Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)
EGF 宿主: 大鼠宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA

Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)
EGF 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)
EGF 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active

Epidermal Growth Factor (EGF) protein
EGF 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS

应用备注

Application Note: EGF Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-EGF in immunological assays.
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5.0 mL

浓度

0.1 mg/mL

缓冲液

Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None

储存液

Without preservative

储存条件

-20 °C

储存方法

Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

有效期

6 months
Khan, Park, Fischer, Dong, Lungjangwa, Jimenez, Casalena, Chew, Dietmann, Auld, Jaenisch, Theunissen: "Probing the signaling requirements for naive human pluripotency by high-throughput chemical screening." in: Cell reports, Vol. 35, Issue 11, pp. 109233, (2022) (PubMed).

Dong, Beltcheva, Gontarz, Zhang, Popli, Fischer, Khan, Park, Yoon, Xing, Kommagani, Wang, Solnica-Krezel, Theunissen: "Derivation of trophoblast stem cells from naïve human pluripotent stem cells." in: eLife, Vol. 9, (2021) (PubMed).
抗原

EGF (Epidermal Growth Factor (EGF))

别名

EGF

背景

Synonyms: Epidermal growth factor, Urogastrone
Background: EGF (Epidermal Growth Factor) protein, of the superfamily with the same name, is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. It is a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4 (HOMG4). Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants. This protein exists in the membrane as a single-pass type I membrane protein, and contains 9 EGF-like domains as well as 9 LDL-receptor class B repeats. It is significantly expressed in kidney, salivary gland, cerebrum and prostate.

UniProt

P01133

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation