(2 references)
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- 抗原 See all EGF 蛋白
- EGF (Epidermal Growth Factor (EGF))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- Supplier Product No.
- 009-001-c26
- Supplier
- Rockland
- 原理
- EGF Human Recombinant Epidermal Growth Factor
- 纯化方法
- Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.
- 纯度
- 95,00%
- 内毒素水平
- Measured by LAL is 0.5 EU/μg protein.
- Biological Activity Comment
- The ED50, calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells, is 30-40 pg/ml.
- Top Product
- Discover our top product EGF 蛋白
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Epidermal Growth Factor (EGF) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Epidermal Growth Factor (EGF) proteinEGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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- 应用备注
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Application Note: EGF Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-EGF in immunological assays.
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5.0 mL
- 浓度
- 0.1 mg/mL
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- EGF (Epidermal Growth Factor (EGF))
- 别名
- EGF (EGF 产品)
- 背景
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Synonyms: Epidermal growth factor, Urogastrone
Background: EGF (Epidermal Growth Factor) protein, of the superfamily with the same name, is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. It is a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4 (HOMG4). Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants. This protein exists in the membrane as a single-pass type I membrane protein, and contains 9 EGF-like domains as well as 9 LDL-receptor class B repeats. It is significantly expressed in kidney, salivary gland, cerebrum and prostate.
- UniProt
- P01133
- 途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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