Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Aspartate aminotransferase 1, Transaminase A, GIG18, Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase cytoplasmic, GIG 18, GIG18, Glutamate oxaloacetate transaminase1, Glutamic oxaloacetic transaminase 1 soluble, GOT 1, Growth inhibiting protein 18, SGOT, Transaminase,. NCBI no.: NP_002070