HNMT Protein (AA 1-292) (His tag)
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- 抗原 See all HNMT 蛋白
- HNMT (Histamine N-Methyltransferase (HNMT))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-292
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This HNMT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA
- 纯度
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C
- Top Product
- Discover our top product HNMT 蛋白
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Histamine N-Methyltransferase (HNMT) (AA 1-292) protein (His tag)
HNMT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Histamine N-Methyltransferase (HNMT) (AA 1-51) protein (GST tag)HNMT 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Histamine N-Methyltransferase (HNMT) (AA 184-292) protein (GST tag)HNMT 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-Hcl ( pH 8.0) buffer containing 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- HNMT (Histamine N-Methyltransferase (HNMT))
- 别名
- HNMT (HNMT 产品)
- 别名
- HMT Protein, HNMT-S1 Protein, HNMT-S2 Protein, hmt Protein, hnmt Protein, hnmt-s1 Protein, hnmt-s2 Protein, zgc:100917 Protein, HNMT Protein, 1500031F01Rik Protein, AI788969 Protein, HMT A Protein, hnmt-a Protein, hnmt-b Protein, histamine N-methyltransferase Protein, histamine N-methyltransferase S homeolog Protein, histamine N-methyltransferase-like Protein, histamine N-methyltransferase L homeolog Protein, HNMT Protein, hnmt.S Protein, hnmt Protein, Hnmt Protein, LOC100228106 Protein, hnmt.L Protein
- 背景
- HNMT also known as Histamine N-methyltransferase. HNMT is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT inactivates histamine by N-methylation. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme histamine N-methyltransferase (HNMT) in bronchus, kidney, and the central nervous system. It plays an important role in degrading histamine and in regulating the airway response to histamine. Recombinant human HNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 37.4 kDa (328aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_008826
- UniProt
- P50135
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