UGDH Protein (AA 1-493) (His tag)
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- 抗原 See all UGDH 蛋白
- UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-493
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This UGDH protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMVEIKKI CCIGAGYVGG PTCSVIAHMC PEIRVTVVDV NEARINAWNS PTLPIYEPGL KEVVESCRGK NLFFSTNIDD AIREADLVFI SVNTPTKTYG MGKGRAADLK YIEACARRIV QNSNGYKIVT EKSTVPVRAA ESIRRIFDAN TKPNLNLQVL SNPEFLAEGT AIKDLKNPDR VLIGGDETPE GQKAVRALCA VYEHWVPKEK ILTTNTWSSE LSKLAANAFL AQRISSINSI SALCEATGAD VEEVATAIGM DQRIGNKFLK ASVGFGGSCF QKDVLNLVYL CEALNLPEVA RYWQQVIDMN DYQRRRFASR IIDSLFNTVT DKKIAILGFA FKKDTGDTRE SSSIYISKYL MDEGAHLHIY DPKVPREQIV VDLSHPGVSA DDQVSRLVTI SKDPYEACDG AHALVICTEW DMFKELDYER IHKKMLKPAF IFDGRRVLDG LHSELQTIGF QIETIGKKVS SKRIPYTPGE IPKFSLQDPP NKKPKV
- 纯度
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C
- Top Product
- Discover our top product UGDH 蛋白
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UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494) protein (His tag)
UGDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE SDS
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494) protein (GST tag)UGDH 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 395-494) protein (GST tag)UGDH 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-493) protein (His tag)UGDH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494) protein (Strep Tag)Crystallography grade UGDH 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494), (full length) protein (His-SUMO Tag)UGDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
UDP-Glucose 6-Dehydrogenase (UGDH) protein (Myc-DYKDDDDK Tag)UGDH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM MES buffer ( pH 5.0) containing 20 % glycerol, 150 mM NaCl, 1 mM EDTA
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))
- 别名
- Ugdh (UGDH 产品)
- 别名
- 8310 Protein, BG01214 Protein, CG10072 Protein, Dmel\\CG10072 Protein, Sgl Protein, UDP-GlcDH Protein, UDPGDH Protein, XIII Protein, kiwi Protein, l(3)05007 Protein, l(3)08310 Protein, l(3)10503 Protein, l(3)65CEd Protein, l(3)SG9 Protein, ska Protein, sl Protein, ik:tdsubc_2a8 Protein, tdsubc_2a8 Protein, xx:tdsubc_2a8 Protein, gdh Protein, ugd Protein, ugdh Protein, ugdhb Protein, xugdh Protein, udpgdh Protein, udpg Protein, ugdha Protein, BA5434 Protein, SCBAC19G2.07 Protein, AGR_L_1413 Protein, udg Protein, GDH Protein, UGD Protein, Udpgdh Protein, sugarless Protein, UDP-glucose 6-dehydrogenase Protein, UDP-glucose 6-dehydrogenase L homeolog Protein, UDP-glucose 6-dehydrogenase S homeolog Protein, UDP-glucose dehydrogenase WcvB Protein, UDP-glucose dehydrogenase Protein, sgl Protein, ugdh Protein, ugdh.L Protein, UGDH Protein, ugdh.S Protein, hasB Protein, ugdH Protein, ugd Protein, SCO3052 Protein, wcvB Protein, CNL06460 Protein, Ugdh Protein
- 背景
- Ugdh, also known as UDP-glucose 6-dehydrohenase, is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant mouse Ugdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 57.2 kDa (516aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_033492
- UniProt
- O70475
- 途径
- Glycosaminoglycan Metabolic Process
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