MDH2 Protein (AA 25-338) (His tag)
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- 抗原 See all MDH2 蛋白
- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 25-338
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MDH2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C
- Top Product
- Discover our top product MDH2 蛋白
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Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) protein (His tag)
MDH2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) protein (His-IF2DI Tag)MDH2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 1-338) protein (GST tag)MDH2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 134-246) protein (GST tag)MDH2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) (Active) protein (His tag)MDH2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In Phosphate buffer saline ( pH 7.4) containing 20 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
- 别名
- Mdh2 (MDH2 产品)
- 别名
- Mdh2b Protein, m-mdh Protein, mdh2 Protein, mor1 Protein, MDH-2 Protein, Mdh Protein, wu:fj48c08 Protein, wu:fj55d06 Protein, zgc:64133 Protein, M-MDH Protein, MDH Protein, MGC:3559 Protein, MOR1 Protein, MMDH Protein, Mdh-2 Protein, Mor-1 Protein, Mor1 Protein, malate dehydrogenase 2 S homeolog Protein, malate dehydrogenase 2 Protein, Malate dehydrogenase 2 Protein, malate dehydrogenase Protein, malate dehydrogenase 2, NAD (mitochondrial) Protein, mdh2.S Protein, MDH2 Protein, Mdh2 Protein, MDH4 Protein, mdh2 Protein
- 背景
- Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 35.4 kDa (335aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_032643
- UniProt
- P08249
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