PDIA3 Protein (AA 25-505) (His tag)
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- 抗原 See all PDIA3 蛋白
- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 25-505
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PDIA3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMSDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNIE SLVKEYDDNG EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE QFLQEYFDGN LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK AQEDL
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >15 A650/cm/min/mg,obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.
- Top Product
- Discover our top product PDIA3 蛋白
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Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 340-505) protein (His-IF2DI Tag)
PDIA3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 1-505) protein (GST tag)PDIA3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 396-505) protein (GST tag)PDIA3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 25-505) protein (His tag)PDIA3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 25-505) protein (His tag)PDIA3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 25-505) protein (His tag)Crystallography grade PDIA3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 25-505) protein (His tag)Crystallography grade PDIA3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 0.1M NaCl, and 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
- 别名
- PDIA3 (PDIA3 产品)
- 别名
- ER60 Protein, ERp57 Protein, ERp60 Protein, ERp61 Protein, GRP57 Protein, GRP58 Protein, HsT17083 Protein, P58 Protein, PI-PLC Protein, grp-58 Protein, 58kDa Protein, Erp Protein, Grp58 Protein, PDI Protein, PDI-Q2 Protein, PLC[a] Protein, Plca Protein, 1 Protein, 25D3-membrane-associated Protein, sb:cb825 Protein, erp57 Protein, grp58 Protein, pdia3 Protein, protein disulfide isomerase family A member 3 Protein, protein disulfide-isomerase A3 Protein, protein disulfide isomerase associated 3 Protein, protein disulfide isomerase family A, member 3 Protein, protein disulfide isomerase family A member 3 S homeolog Protein, PDIA3 Protein, Tsp_10062 Protein, Pdia3 Protein, pdia3 Protein, pdia3.S Protein
- 背景
- PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant mouse PDIA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 56.8kDa (505aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_031978
- UniProt
- P27773
- 途径
- Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
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