Luciferase Protein (AA 1-311) (His tag)
宿主: Sea Pansy
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % by SDS-PAGE
SDS
Active
1 image
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- 抗原 See all Luciferase 蛋白
- Luciferase
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-311
- 宿主
- Sea Pansy
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Luciferase protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSHMTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ
- 纯度
- > 95 % by SDS-PAGE
- Biological Activity Comment
- Specific activity is >1X109 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH7.5 at 25C.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Luciferase
- Abstract
- Luciferase 产品
- 别名
- hypothetical protein Protein, luciferase Protein, C25G6.3 Protein, Gbro_2186 Protein
- 背景
- LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein. Recombinant renilla reniformis LUC, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 38.5 kDa (335aa) Confirmed by MALDI-TOF
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