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AHCY Protein (AA 1-432) (His tag)

Name: AHCY Protein (AA 1-432) (His tag)
SKU: ABIN6388020
Availability: OutOfStock

AHCY 宿主: 人宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS

SDS-PAGE (SDS) image for Adenosylhomocysteinase (AHCY) (AA 1-432) protein (His tag) (ABIN6388020) (AHCY Protein (AA 1-432) (His tag))

SDS

1 image

产品编号 ABIN6388020

发货至: 中国

Datasheet as PDF

抗原 See all AHCY 蛋白

AHCY (Adenosylhomocysteinase (AHCY))

蛋白类型

Recombinant

产品特性

AA 1-432
宿主
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资源
61

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Baculovirus infected Insect Cells

标记

This AHCY protein is labelled with His tag.

应用范围

SDS-PAGE (SDS)

序列

ADLMSDKLPY KVADIGLAAW GRKALDIAEN EMPGLMRMRE RYSASKPLKG ARIAGCLHMT VETAVLIETL VTLGAEVQWS SCNIFSTQDH AAAAIAKAGI PVYAWKGETD EEYLWCIEQT LYFKDGPLNM ILDDGGDLTN LIHTKYPQLL PGIRGISEET TTGVHNLYKM MANGILKVPA INVNDSVTKS KFDNLYGCRE SLIDGIKRAT DVMIAGKVAV VAGYGDVGKG CAQALRGFGA RVIITEIDPI NALQAAMEGY EVTTMDEACQ EGNIFVTTTG CIDIILGRHF EQMKDDAIVC NIGHFDVEID VKWLNENAVE KVNIKPQVDR YRLKNGRRII LLAEGRLVNL GCAMGHPSFV MSNSFTNQVM AQIELWTHPD KYPVGVHFLP KKLDEAVAEA HLGKLNVKLT KLTEKQAQYL GMSCDGPFKP DHYRYHHHHH H

纯度

> 90 % by SDS - PAGE

内毒素水平

< 1.0 EU per 1 microgram of protein (determined by LAL method)

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Adenosylhomocysteinase (AHCY) (AA 1-432) protein (His tag)
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Adenosylhomocysteinase (AHCY) (AA 1-432) protein (His tag)
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Adenosylhomocysteinase (AHCY) (AA 2-432) protein (His tag)
Crystallography grade AHCY 宿主: 人宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys

Adenosylhomocysteinase (AHCY) (AA 2-432) protein (His tag)
Crystallography grade AHCY 宿主: 小鼠宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys

Adenosylhomocysteinase (AHCY) (AA 2-432) protein (His tag)
AHCY 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, Imm, PC

Adenosylhomocysteinase (AHCY) (AA 2-432) protein (His-SUMO Tag)
AHCY 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS

Adenosylhomocysteinase (AHCY) (AA 1-448), (full length) protein (His tag)
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Adenosylhomocysteinase (AHCY) protein (Myc-DYKDDDDK Tag)
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Adenosylhomocysteinase (AHCY) (AA 2-432) (Active) protein (His tag)
AHCY 宿主: 人宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

浓度

0.25 mg/mL

缓冲液

Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

储存条件

4 °C,-20 °C,-80 °C

储存方法

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
抗原

AHCY (Adenosylhomocysteinase (AHCY))

别名

AHCY (AHCY 产品)

别名

sahh Protein, PSPTO5068 Protein, Ahcy Protein, SAHH Protein, adoHcyase Protein, ahcy Protein, AA987153 Protein, AL024110 Protein, CuBP Protein, D150 Protein, cb1079 Protein, hm:zeh1173 Protein, hm:zeh1364 Protein, wu:fj67b02 Protein, adenosylhomocysteinase Protein, Adenosylhomocysteinase Protein, S-adenosylhomocysteine hydrolase Protein, adenosylhomocysteinase S homeolog Protein, AHCY Protein, ahcy Protein, ahcY Protein, MMAH_RS02605 Protein, Srot_2217 Protein, Ndas_3755 Protein, Deba_0936 Protein, Palpr_2392 Protein, Calni_0569 Protein, LOC100282150 Protein, Intca_2510 Protein, Marky_0392 Protein, Desac_1394 Protein, Tc00.1047053511229.50 Protein, Tc00.1047053511589.200 Protein, Tb11.01.1350 Protein, LMJF_36_3910 Protein, MCYG_06244 Protein, Ahcy Protein, ahcy.S Protein

背景

AHCY, also known as adenosylhomocysteinase isoform 1, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYs activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant human AHCY protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

分子量

48.8kDa (441aa) 40-57KDa (SDS-PAGE under reducing conditions.)

NCBI登录号

NP_000678

UniProt

P23526