ECHS1 Protein (AA 28-290) (His tag)
-
- 抗原 See all ECHS1 蛋白
- ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 28-290
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This ECHS1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.
- Top Product
- Discover our top product ECHS1 蛋白
-
Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1) (AA 13-290) protein (GST tag)
ECHS1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1) (AA 28-127) protein (GST tag)ECHS1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1) (AA 28-290) protein (His tag)Crystallography grade ECHS1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1) (AA 28-290) protein (His tag)Crystallography grade ECHS1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1) protein (Myc-DYKDDDDK Tag)ECHS1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Bioactivity Validated
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 100 mM NaCl
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))
- 别名
- ECHS1 (ECHS1 产品)
- 别名
- SCEH Protein, cOR6not Protein, fj55e05 Protein, si:zc217g15.1 Protein, wu:fj55e05 Protein, C80529 Protein, enoyl-CoA hydratase, short chain, 1, mitochondrial L homeolog Protein, enoyl-CoA hydratase, short chain 1 Protein, enoyl CoA hydratase, short chain, 1, mitochondrial Protein, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial Protein, echs1.L Protein, ECHS1 Protein, Echs1 Protein, echs1 Protein
- 背景
- Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 30.6 kDa (284aa), confirmed by MALDI-TOF
- 途径
- Monocarboxylic Acid Catabolic Process
-
