PGP Protein (AA 1-321) (His tag)
-
- 抗原 See all PGP 蛋白
- PGP (Phosphoglycolate Phosphatase (PGP))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-321
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This PGP protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product PGP 蛋白
-
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (His tag)
PGP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (Strep Tag)Crystallography grade PGP 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (Strep Tag)Crystallography grade PGP 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycolate Phosphatase (PGP) protein (Myc-DYKDDDDK Tag)PGP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Bioactivity Validated
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- PGP (Phosphoglycolate Phosphatase (PGP))
- 别名
- PGP (PGP 产品)
- 别名
- RGD1307773 Protein, cb241 Protein, sb:cb241 Protein, wu:fb92c03 Protein, wu:fi40b02 Protein, zgc:56011 Protein, 1700012G19Rik Protein, AI481330 Protein, phosphoglycolate phosphatase Protein, Pgp Protein, PGP Protein, pgp Protein
- 背景
- Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 36.5 kDa (345aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_001035830
- UniProt
- A6NDG6
-
