PPIF Protein (AA 30-206) (His tag)
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- 抗原 See all PPIF 蛋白
- PPIF (Peptidylprolyl Isomerase F (PPIF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 30-206
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PPIF protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS
- 纯度
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
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- Discover our top product PPIF 蛋白
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Peptidylprolyl Isomerase F (PPIF) (AA 30-207) protein
PPIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS PAGE SDS
Peptidylprolyl Isomerase F (PPIF) (AA 20-206) protein (His tag)PPIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Peptidylprolyl Isomerase F (PPIF) (AA 30-207) (Active) proteinPPIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Peptidylprolyl Isomerase F (PPIF) (AA 120-207) protein (GST tag)PPIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peptidylprolyl Isomerase F (PPIF) (AA 1-207) protein (GST tag)PPIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peptidylprolyl Isomerase F (PPIF) (AA 30-207) protein (His tag)Crystallography grade PPIF 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Peptidylprolyl Isomerase F (PPIF) (AA 30-206) protein (His tag)Crystallography grade PPIF 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PPIF (Peptidylprolyl Isomerase F (PPIF))
- 别名
- Ppif (PPIF 产品)
- 别名
- CYP3 Protein, CyP-M Protein, Cyp-D Protein, CypD Protein, CyP-D Protein, ppif Protein, wu:fd61c06 Protein, zgc:101753 Protein, AW457192 Protein, CyP-F Protein, PPIase Protein, Cyp-40 Protein, cypf Protein, peptidylprolyl isomerase F Protein, peptidylprolyl isomerase Fa Protein, peptidylprolyl isomerase F (cyclophilin F) Protein, peptidylprolyl isomerase D Protein, peptidylprolyl isomerase F L homeolog Protein, PPIF Protein, ppifa Protein, Ppif Protein, Ppid Protein, ppif Protein, ppif.L Protein
- 背景
- Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 21.2Da (200aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_758443
- UniProt
- P29117
- 途径
- Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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