Glucuronidase beta Protein (AA 23-651) (His tag)
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- 抗原 See all Glucuronidase beta (GUSB) 蛋白
- Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-651
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This Glucuronidase beta protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH
- 纯度
- > 90 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH6.0
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- Discover our top product GUSB 蛋白
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Glucuronidase, beta (GUSB) (AA 22-651) protein (GST tag)
GUSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glucuronidase, beta (GUSB) (AA 23-651) protein (His tag)Crystallography grade GUSB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glucuronidase, beta (GUSB) (AA 23-648) protein (His tag)Crystallography grade GUSB 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glucuronidase, beta (GUSB) proteinGUSB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
- 别名
- GUSB (GUSB 产品)
- 别名
- GUSB Protein, beta-GUS Protein, gus Protein, BG Protein, MPS7 Protein, AI747421 Protein, Gur Protein, Gus Protein, Gus-r Protein, Gus-s Protein, Gus-t Protein, Gus-u Protein, Gut Protein, asd Protein, g Protein, Ac2-223 Protein, si:ch211-160e1.7 Protein, si:ct573103.7 Protein, glucuronidase beta Protein, beta-D-glucuronidase Protein, beta-glucuronidase Protein, beta glucuronidase Protein, glucuronidase, beta Protein, GUSB Protein, bglR Protein, SSO_RS14735 Protein, Glu Protein, Gusb Protein, gusb Protein
- 背景
- GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 73.4kDa (635aa) 70-100KDa (SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_000172
- UniProt
- P08236
- 途径
- Glycosaminoglycan Metabolic Process
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