HPRT1 Protein (AA 1-218) (His tag)
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- 抗原 See all HPRT1 蛋白
- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-218
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This HPRT1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate(GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
- Top Product
- Discover our top product HPRT1 蛋白
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Hypoxanthine phosphoribosyltransferase 1 (HPRT1) (AA 1-218) protein (GST tag)
HPRT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) (AA 2-218) protein (His tag)Crystallography grade HPRT1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) protein (Myc-DYKDDDDK Tag)HPRT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing, 20 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
- 别名
- HPRT (HPRT1 产品)
- 别名
- HGPRT Protein, HPRT Protein, C81579 Protein, HPGRT Protein, Hprt1 Protein, Hgprtase Protein, Hprt Protein, id:ibd1344 Protein, id:ibd5108 Protein, wu:fc10g09 Protein, zgc:56221 Protein, zgc:86608 Protein, hgprt Protein, hprt Protein, prtfdc1 Protein, hprt1 Protein, hprt1l Protein, zgc:55561 Protein, zgc:86771 Protein, hypoxanthine phosphoribosyltransferase 1 Protein, hypoxanthine guanine phosphoribosyl transferase Protein, hypoxanthine phosphoribosyltransferase 1 L homeolog Protein, phosphoribosyl transferase domain containing 1 Protein, HPRT1 Protein, Hprt Protein, Hprt1 Protein, hprt1 Protein, hprt1.L Protein, prtfdc1 Protein
- 背景
- Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 26.7 kDa (238aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_000185
- UniProt
- P00492
- 途径
- Ribonucleoside Biosynthetic Process
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