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GUK1 Protein (AA 1-197) (His tag)

Name: GUK1 Protein (AA 1-197) (His tag)
SKU: ABIN6387883
Availability: OutOfStock

GUK1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active

产品编号 ABIN6387883

发货至: 中国

Datasheet as PDF

抗原 See all GUK1 蛋白

GUK1 (Guanylate Kinase 1 (GUK1))

蛋白类型

Recombinant

生物活性

Active

产品特性

AA 1-197
宿主
6

2

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人
资源
45

4

2

1
大肠杆菌（E. Coli）

标记

This GUK1 protein is labelled with His tag.

应用范围

SDS-PAGE (SDS)

序列

MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

纯度

> 95 % by SDS - PAGE

Biological Activity Comment

Specific activity is > 90 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

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Guanylate Kinase 1 (GUK1) (AA 2-198) protein (His tag)
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应用备注

Optimal working dilution should be determined by the investigator.

说明

Bioactivity Validated

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20 % glycerol

储存条件

4 °C,-20 °C,-80 °C

储存方法

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
抗原

GUK1 (Guanylate Kinase 1 (GUK1))

别名

GUK1 (GUK1 产品)

别名

GMK Protein, AA409738 Protein, AL033299 Protein, AL033300 Protein, AV026611 Protein, Gmk Protein, Guk-1 Protein, guk1 Protein, zgc:73128 Protein, zgc:73217 Protein, gmk Protein, GUK1 Protein, AGK1 Protein, T11A7.23 Protein, guanylate kinase 1 Protein, DDBDRAFT_0215291 Protein, DDBDRAFT_0230100 Protein, DDB_0215291 Protein, DDB_0230100 Protein, sb:cb295 Protein, zgc:86776 Protein, guanylate kinase 1 Protein, guanylate kinase (GUK1) Protein, guanylate kinase Protein, guanylate kinase 1b Protein, guanylate kinase 1 L homeolog Protein, guanylate kinase 1a Protein, GUK1 Protein, Guk1 Protein, guk1b Protein, guk1 Protein, guk1.L Protein, GK-1 Protein, gmk Protein, gmkA Protein, Mrub_2093 Protein, Arnit_0783 Protein, Ndas_3097 Protein, Mesil_1319 Protein, Slip_0854 Protein, Olsu_0986 Protein, Acear_1451 Protein, AOR_1_2886174 Protein, guk1a Protein

背景

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-  kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.

分子量

36kDa (335aa), confirmed by MALDI-TOF

NCBI登录号

NP_002037

UniProt

P04406

途径

Nucleotide Phosphorylation, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process