PGAM2 Protein (AA 1-254) (His tag)
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- 抗原 See all PGAM2 蛋白
- PGAM2 (phosphoglycerate Mutase 2 (Muscle) (PGAM2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-254
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PGAM2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >100units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
- Top Product
- Discover our top product PGAM2 蛋白
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phosphoglycerate Mutase 2 (Muscle) (PGAM2) (AA 1-253) protein (His tag)
PGAM2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
phosphoglycerate Mutase 2 (Muscle) (PGAM2) (AA 1-253) protein (GST tag)PGAM2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
phosphoglycerate Mutase 2 (Muscle) (PGAM2) protein (Myc-DYKDDDDK Tag)PGAM2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PGAM2 (phosphoglycerate Mutase 2 (Muscle) (PGAM2))
- 别名
- PGAM2 (PGAM2 产品)
- 别名
- zgc:63876 Protein, GSD10 Protein, PGAM-M Protein, PGAMM Protein, D14Mgh1 Protein, Pgmut Protein, phosphoglycerate mutase 2 Protein, phosphoglycerate mutase 2 (muscle) Protein, phosphoglycerate mutase 2 L homeolog Protein, Pgam2 Protein, PGAM2 Protein, pgam2 Protein, pgam2.L Protein
- 背景
- PGAM2, also known as phosphoglycerate mutase 2, belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 30.9 kDa (273aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_000281
- UniProt
- P15259
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