PGAM1 Protein (AA 1-254) (His tag)
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- 抗原 See all PGAM1 蛋白
- PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-254
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PGAM1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
- Top Product
- Discover our top product PGAM1 蛋白
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phosphoglycerate Mutase 1 (Brain) (PGAM1) (AA 1-254) protein (GST tag)
PGAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
phosphoglycerate Mutase 1 (Brain) (PGAM1) (AA 79-154) protein (His-IF2DI Tag)PGAM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
phosphoglycerate Mutase 1 (Brain) (PGAM1) (AA 1-254) protein (His tag)PGAM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
phosphoglycerate Mutase 1 (Brain) (PGAM1) (AA 1-254) protein (His tag)PGAM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
phosphoglycerate Mutase 1 (Brain) (PGAM1) (AA 2-254) protein (His tag)Crystallography grade PGAM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
phosphoglycerate Mutase 1 (Brain) (PGAM1) protein (Myc-DYKDDDDK Tag)PGAM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))
- 别名
- PGAM1 (PGAM1 产品)
- 别名
- PGAM-B Protein, PGAMA Protein, Pgmut Protein, MGC81450 Protein, 2310050F24Rik Protein, Pgam-1 Protein, pgam1 Protein, wu:fb81h05 Protein, fd15c02 Protein, pgam1lb Protein, wu:fd15c02 Protein, zgc:63722 Protein, phosphoglycerate mutase 1 Protein, phosphoglycerate mutase 1 L homeolog Protein, phosphoglycerate mutase 1a Protein, phosphoglycerate mutase 1b Protein, PGAM1 Protein, Pgam1 Protein, pgam1.L Protein, pgam1 Protein, pgam1a Protein, pgam1b Protein
- 背景
- PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 31.4 kDa (278aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_075907
- UniProt
- Q9DBJ1
- 途径
- Regulation of Carbohydrate Metabolic Process
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