GLO1 Protein (AA 1-184) (His tag)
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- 抗原 See all GLO1 蛋白
- GLO1 (Glyoxalase I (GLO1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-184
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This GLO1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.
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- Discover our top product GLO1 蛋白
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Glyoxalase I (GLO1) (AA 1-184) protein
GLO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Glyoxalase I (GLO1) (AA 2-184) protein (His tag)GLO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyoxalase I (GLO1) (AA 1-184) (Active) proteinGLO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Glyoxalase I (GLO1) (AA 1-184) protein (GST tag)GLO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyoxalase I (GLO1) (AA 1-98) protein (GST tag)GLO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyoxalase I (GLO1) protein (His tag)GLO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Glyoxalase I (GLO1) (AA 2-184) protein (His tag)Crystallography grade GLO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- GLO1 (Glyoxalase I (GLO1))
- 别名
- Glo1 (GLO1 产品)
- 别名
- GLOD1 Protein, GLYI Protein, 0610009E22Rik Protein, 1110008E19Rik Protein, 2510049H23Rik Protein, AW550643 Protein, GLY1 Protein, Glo-1 Protein, Glo-1r Protein, Glo-1s Protein, Glo1-r Protein, Glo1-s Protein, Qglo Protein, cb554 Protein, zgc:66035 Protein, wu:fb82g09 Protein, glod1 Protein, glyi Protein, glo1 Protein, GLO1 Protein, ATGLX1 Protein, F12F1.32 Protein, F12F1_32 Protein, GLYOXALASE I Protein, glyoxalase I homolog Protein, trypanothione-dependent glyoxalase I Protein, glyoxalase I Protein, glyoxalase 1 Protein, glyoxalase 1 L homeolog Protein, glyoxalase 1 S homeolog Protein, lactoylglutathione lyase Protein, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein Protein, GLO1 Protein, Glo1 Protein, glo1 Protein, glo1.L Protein, glo1.S Protein, GST3 Protein, GLX1 Protein, STY1687 Protein, Bcen_2094 Protein, gloA Protein
- 背景
- Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 21.8kDa (192aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI登录号
- NP_079650
- UniProt
- Q9CPU0
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