PGK2 Protein (AA 1-417) (His tag)
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- 抗原 See all PGK2 蛋白
- PGK2 (Phosphoglycerate Kinase 2 (PGK2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-417
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PGK2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product PGK2 蛋白
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Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (His tag)
PGK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (GST tag)PGK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Phosphoglycerate Kinase 2 (PGK2) (AA 268-339) protein (GST tag)PGK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (Strep Tag)Crystallography grade PGK2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycerate Kinase 2 (PGK2) (AA 2-417) protein (His tag)Crystallography grade PGK2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Phosphoglycerate Kinase 2 (PGK2) protein (Myc-DYKDDDDK Tag)PGK2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT, 0.1M NaCl
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PGK2 (Phosphoglycerate Kinase 2 (PGK2))
- 别名
- PGK2 (PGK2 产品)
- 别名
- PGKB Protein, PGKPS Protein, dJ417L20.2 Protein, Pgk-2 Protein, Tcp-2 Protein, PGK2 Protein, phosphoglycerate kinase 2 Protein, PGK2 Protein, Pgk2 Protein, cbbK Protein
- 背景
- PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 46.9 kDa (437aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_620061
- UniProt
- P07205
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