PGK1 Protein (AA 1-417) (His tag)
-
- 抗原 See all PGK1 蛋白
- PGK1 (Phosphoglycerate Kinase 1 (PGK1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-417
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This PGK1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product PGK1 蛋白
-
Phosphoglycerate Kinase 1 (PGK1) (AA 307-417) protein (His-IF2DI Tag)
PGK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) protein (His tag)PGK1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) protein (His tag)PGK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) protein (GST tag)PGK1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Phosphoglycerate Kinase 1 (PGK1) (AA 321-417) protein (GST tag)PGK1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Bioactivity Validated
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris ( pH 8.0), containing 10 % Glycerol, 1 mM DTT.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- PGK1 (Phosphoglycerate Kinase 1 (PGK1))
- 别名
- PGK1 (PGK1 产品)
- 别名
- MIG10 Protein, PGKA Protein, Pgk-1 Protein, Pgk Protein, PGK Protein, wu:fd59b07 Protein, wu:fj36g06 Protein, zgc:56252 Protein, zgc:77899 Protein, PGK1 Protein, phosphoglycerate kinase 1 Protein, pgk2 Protein, pgka Protein, phosphoglycerate kinase 1 Protein, phosphoglycerate kinase 1 L homeolog Protein, PGK1 Protein, Pgk1 Protein, pgk1 Protein, LOC100348124 Protein, pgk1.L Protein
- 背景
- Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 46.8 kDa (437aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_000282
- UniProt
- P00558
- 途径
- Cellular Glucan Metabolic Process
-
