PGD Protein (AA 1-483) (His tag)
-
- 抗原 See all PGD 蛋白
- PGD (Phosphogluconate Dehydrogenase (PGD))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-483
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This PGD protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA
- 纯度
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.
- Top Product
- Discover our top product PGD 蛋白
-
Phosphogluconate Dehydrogenase (PGD) (AA 33-259) protein (His tag)
PGD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Phosphogluconate Dehydrogenase (PGD) (AA 1-483) protein (GST tag)PGD 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Phosphogluconate Dehydrogenase (PGD) (AA 4-483), (partial) protein (GST tag)PGD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Bioactivity Validated
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 0.1M Nacl, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- PGD (Phosphogluconate Dehydrogenase (PGD))
- 别名
- PGD (PGD 产品)
- 别名
- 6PGD Protein, 6-PGD Protein, 6-Pgd Protein, 6-pgd Protein, 6PGDH Protein, 6Pgd Protein, 6Pgdh Protein, 6pgdh Protein, A7 Protein, CG3724 Protein, Dmel\\CG3724 Protein, EG:87B1.4 Protein, N1 Protein, PGD Protein, Pdg Protein, l(1)2Dc Protein, l(1)2De Protein, l(1)A7 Protein, l(1)G0385 Protein, l(1)N1 Protein, l(1)N3[90] Protein, l(1)Pgd Protein, l(1)Pgd-A Protein, l35 Protein, pgd Protein, 0610042A05Rik Protein, AU019875 Protein, C78335 Protein, fi03d03 Protein, zgc:55585 Protein, wu:fi03d03 Protein, zgc:111848 Protein, 6-PGDH Protein, GND1b Protein, Pgd Protein, Cc2-27 Protein, phosphogluconate dehydrogenase Protein, Phosphogluconate dehydrogenase Protein, phosphogluconate dehydrogenase L homeolog Protein, 6-phosphogluconate dehydrogenase, decarboxylating Protein, 6-phosphogluconate dehydrogenase, decarboxylating-like Protein, PGD Protein, Pgd Protein, pgd.L Protein, pgd Protein, GND1a|GND1b Protein, Thal_1580 Protein, GY4MC1_3101 Protein, Odosp_2189 Protein, Marky_1024 Protein, gnd Protein, LOC101451876 Protein
- 背景
- PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 55.3 kDa (503aa)
- NCBI登录号
- NP_002622
- UniProt
- P52209
-
