NMNAT1 Protein (AA 115-270) (His tag)
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- 抗原 See all NMNAT1 蛋白
- NMNAT1 (Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 115-270
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NMNAT1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQ SIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product NMNAT1 蛋白
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Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 1-279) protein (His tag)
NMNAT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 1-285) protein (His tag)NMNAT1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 1-279) protein (GST tag)NMNAT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 51-150) protein (GST tag)NMNAT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) protein (Myc-DYKDDDDK Tag)NMNAT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1 mM DTT. 1 mM EDTA
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- NMNAT1 (Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1))
- 别名
- NMNAT1 (NMNAT1 产品)
- 别名
- id:ibd5068 Protein, im:7144541 Protein, zgc:110243 Protein, LCA9 Protein, NMNAT Protein, PNAT1 Protein, 2610529L11Rik Protein, 5730441G13Rik Protein, D4Cole1e Protein, nmnat Protein, nicotinamide mononucleotide adenylyltransferase 1 Protein, nicotinamide nucleotide adenylyltransferase 1 Protein, CpipJ_CPIJ015320 Protein, PTRG_06486 Protein, nmnat1 Protein, NMNAT1 Protein, Nmnat1 Protein
- 背景
- NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 36.0 kDa (315aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_073624
- UniProt
- Q9HAN9
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