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NARS Protein (AA 1-548) (His tag)
NARS
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 80 % by SDS - PAGE
SDS
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抗原
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NARS
(Asparaginyl-tRNA Synthetase (NARS))
蛋白类型
Recombinant
产品特性
AA 1-548
宿主
All origins for NARS 蛋白
人
资源
All sources for NARS 蛋白
大肠杆菌(E. Coli)
标记
This NARS protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
序列
MGSSHHHHHH SSGLVPRGSH MGSMVLAELY VSDREGSDAT GDGTKEKPFK TGLKALMTVG KEPFPTIYVD SQKENERWNV ISKSQLKNIK KMWHREQMKS ESREKKEAED SLRREKNLEE AKKITIKNDP SLPEPKCVKI GALEGYRGQR VKVFGWVHRL RRQGKNLMFL VLRDGTGYLQ CVLADELCQC YNGVLLSTES SVAVYGMLNL TPKGKQAPGG HELSCDFWEL IGLAPAGGAD NLINEESDVD VQLNNRHMMI RGENMSKILK ARSMVTRCFR DHFFDRGYYE VTPPTLVQTQ VEGGATLFKL DYFGEEAFLT QSSQLYLETC LPALGDVFCI AQSYRAEQSR TRRHLAEYTH VEAECPFLTF DDLLNRLEDL VCDVVDRILK SPAGSIVHEL NPNFQPPKRP FKRMNYSDAI VWLKEHDVKK EDGTFYEFGE DIPEAPERLM TDTINEPILL CRFPVEIKSF YMQRCPEDSR LTESVDVLMP NVGEIVGGSM RIFDSEEILA GYKREGIDPT PYYWYTDQRK YGTCPHGGYG LGLERFLTWI LNRYHIRDVC LYPRFVQRCT P
纯度
> 80 % by SDS - PAGE
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
0.25 mg/mL
缓冲液
Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
目标详细情况
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抗原
NARS
(Asparaginyl-tRNA Synthetase (NARS))
别名
NARS (NARS 产品 )
别名
ASNRS Protein, NARS1 Protein, AsnRS Protein, BcDNA:GH06451 Protein, CG10687 Protein, Ca Protein, Dmel\\CG10687 Protein, NRS Protein, l(2)37Ca Protein, l(2)37Ca)E11 Protein, l(2)E11 Protein, 3010001M15Rik Protein, AA960128 Protein, C78150 Protein, LRRGT00113 Protein, fb57a10 Protein, wu:fb57a10 Protein, si:dkey-274m14.2 Protein, BA4802 Protein, asparaginyl-tRNA synthetase Protein, Asparaginyl-tRNA synthetase Protein, asparagine--tRNA ligase Protein, asparaginyl-tRNA synthetase AsnS Protein, asparaginyl-tRNA ligase Protein, asparaginyl-tRNA synthetase 2, mitochondrial Protein, asparagine--tRNA ligase DED81 Protein, asparaginyL-tRNA synthetase Protein, NARS Protein, AsnRS Protein, Nars Protein, nars Protein, asnS Protein, asnC Protein, GYMC10_2250 Protein, Mesil_2042 Protein, Trad_1774 Protein, NARS2 Protein, DED81 Protein, Ilyop_2011 Protein, Palpr_1926 Protein, Ocepr_1116 Protein, Olsu_0392 Protein
背景
NARS also known as asparagine--tRNA ligase, cytoplasmic. This protein acts as an enzyme. It is known to catalyze the following reaction: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. Recombinant human NARS, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
分子量
65.3kDa (571aa)
NCBI登录号
NP_004530
UniProt
O43776
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NARS 蛋白
NARS Protein (AA 1-548) (His tag) (ABIN6387628)