GATC Protein (AA 1-136) (His tag)
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- 抗原 See all GATC 蛋白
- GATC (Glutamyl-tRNA Amidotransferase, Subunit C (GATC))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-136
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GATC protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS
- 纯度
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GATC 蛋白
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Glutamyl-tRNA Amidotransferase, Subunit C (GATC) (AA 1-136) protein (His tag)
GATC 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glutamyl-tRNA Amidotransferase, Subunit C (GATC) (AA 1-136) protein (GST tag)GATC 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glutamyl-tRNA Amidotransferase, Subunit C (GATC) protein (Myc-DYKDDDDK Tag)GATC 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 30 % glycerol, 1 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- GATC (Glutamyl-tRNA Amidotransferase, Subunit C (GATC))
- 别名
- GATC (GATC 产品)
- 别名
- 15E1.2 Protein, RGD1309698 Protein, 2010003O18Rik Protein, gatc Protein, glutamyl-tRNA amidotransferase subunit C Protein, glutamyl-tRNA(Gln) amidotransferase, subunit C Protein, si:rp71-1c23.3 Protein, GATC Protein, Gatc Protein, gatc Protein, si:rp71-1c23.3 Protein
- 背景
- GATC allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 17.5 kDa (159aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI登录号
- NP_789788
- UniProt
- O43716
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