EGLN3 Protein (His tag)
-
- 抗原 See all EGLN3 蛋白
- EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This EGLN3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by using conventional chromatography.
- Top Product
- Discover our top product EGLN3 蛋白
-
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (His tag)
EGLN3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (GST tag)EGLN3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 94-193) protein (GST tag)EGLN3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 2-239) protein (His tag)EGLN3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) protein (Myc-DYKDDDDK Tag)EGLN3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 说明
-
Synonyms: Egl nine homolog 3, HIFPH3, PHD3
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.25 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5 MM DTT, 2 mM EDTA
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
-
- 抗原
- EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))
- 别名
- EGLN3 (EGLN3 产品)
- 别名
- zgc:77019 Protein, wu:fj78a08 Protein, EGLN3 Protein, phd3 Protein, PHD-3 Protein, PHD3 Protein, SM-20 Protein, 2610021G09Rik Protein, AI505553 Protein, AI648162 Protein, Hif-p4h-3 Protein, Phd3 Protein, HIFP4H3 Protein, HIFPH3 Protein, egl-9 family hypoxia-inducible factor 3 Protein, egl-9 family hypoxia inducible factor 3 Protein, egl-9 family hypoxia-inducible factor 3 L homeolog Protein, egln3 Protein, EGLN3 Protein, egln3.L Protein, Egln3 Protein
- 背景
- EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 29.8 kDa (263aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_071356
- 途径
- Positive Regulation of Endopeptidase Activity
-
