IL1A Protein (AA 113-271)
Quick Overview for IL1A Protein (AA 113-271) (ABIN6253323)
抗原
See all IL1A 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 113-271
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原理
- IL-1alpha (human) (rec.)
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特异性
- Human IL-1alpha (aa 113-271).
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产品特性
- Protein. Human IL-1alpha (aa 113-271). Source: HEK 293 cells. Endotoxin content: <0.01EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.
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内毒素水平
- <0.01EU/μg protein (LAL test, Lonza).
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Biological Activity Comment
- The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a specific activity of > 1 x 109units/mg.
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Interleukin 1 alpha (IL1A) (Active) protein
IL1A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 1 alpha (IL1A) (AA 113-271) protein (His tag)IL1A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 1 alpha (IL1A) (AA 113-271) proteinIL1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 1 alpha (IL1A) (AA 113-271) protein (Fc Tag)IL1A 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 1 alpha (IL1A) (AA 113-271) protein (His tag)IL1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Interleukin 1 alpha (IL1A) (AA 115-270) protein (His tag)IL1A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 1 alpha (IL1A) proteinIL1A 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 1 alpha (IL1A) (AA 1-271) protein (GST tag)IL1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 1 alpha (IL1A) (AA 172-271) protein (GST tag)IL1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 1 alpha (IL1A) proteinIL1A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Lot specific
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缓冲液
- Lyophilized from 0.2μm-filtered solution in PBS.
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注意事项
- Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- IL-1alpha
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背景
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Alternate Names/Synonyms: Interleukin-1alpha, IL-1F1, IL-1A
Product Description: The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.
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分子量
- ~19kDa (SDS-PAGE)
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NCBI登录号
- NP_000566
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原
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