SIRT3 Protein (AA 5-257) (His tag)
-
- 抗原 See all SIRT3 蛋白
- SIRT3 (Sirtuin 3 (SIRT3))
- 生物活性
- Active
- 产品特性
- AA 5-257
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This SIRT3 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs). SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Dihydrolipoyl Transacetylase (DLAT) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse DLAT. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DLAT-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and DLAT was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SIRT3 with DLAT.
- Top Product
- Discover our top product SIRT3 蛋白
-
Sirtuin 3 (SIRT3) (AA 1-399) protein (GST tag)
SIRT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 3 (SIRT3) (AA 118-399) protein (His tag)SIRT3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sirtuin 3 (SIRT3) (AA 297-399) protein (GST tag)SIRT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 3 (SIRT3) (AA 127-303) (Active) protein (His tag)SIRT3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Sirtuin 3 (SIRT3) proteinSIRT3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
-
- 应用备注
- Isoelectric Point: 6.4
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- 抗原
- SIRT3 (Sirtuin 3 (SIRT3))
- Abstract
- SIRT3 产品
- 别名
- zgc:158774 Protein, sirtuin Protein, SIR2L3 Protein, 2310003L23Rik Protein, AI848213 Protein, Sir2l3 Protein, sirtuin 3 Protein, sirt3 Protein, SIRT3 Protein, Sirt3 Protein
- 背景
- Alternative Names: SIR2L3, Silent Mating Type Information Regulation 2 Homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, Regulatory protein SIR2 homolog 3
- 分子量
- 16&29kDa
- UniProt
- Q8R104
-
