ARG Protein (AA 1-322) (His tag)
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- 抗原 See all ARG 蛋白
- ARG (Arginase (ARG))
- 生物活性
- Active
- 产品特性
- AA 1-322
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ARG protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Arg and recombinant human UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of Arg with UCHL5.
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- Discover our top product ARG 蛋白
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Arginase (ARG) (AA 1-323) (Active) protein (His tag)
ARG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Arginase (ARG) (AA 1-323) (Active) protein (His tag)ARG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Arginase (ARG) proteinARG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- Isoelectric Point: 6.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- ARG (Arginase (ARG))
- Abstract
- ARG 产品
- 别名
- CG18104 Protein, Dmel\\CG18104 Protein, EG:171D11.4 Protein, EG:65F1.3 Protein, SI:zC146F4.4 (novel protein with NUDIX domain) Protein, si:ch211-146f4.3 Protein, Tb08.26N11.490 Protein, rocF Protein, NV10213 Protein, argi1 Protein, arginase Protein, arginase 1 L homeolog Protein, arginase 1 Protein, Arginase-1 Protein, arg Protein, arg1.L Protein, arg1 Protein, ARGAH1 Protein, BP0538 Protein, rocF Protein, SAS2066 Protein, RR_RS05730 Protein, CND03500 Protein, CNG00550 Protein, Tb927.8.2020 Protein, PGTG_16455 Protein, LOC100123155 Protein, argi1 Protein
- 背景
- Alternative Names: ARG1, Arginase I, Liver Arginase
- 分子量
- 37kDa
- UniProt
- P05089
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