Arylsulfatase B Protein (ARSB) (AA 81-533) (His tag)
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- 抗原 See all Arylsulfatase B (ARSB) 蛋白
- Arylsulfatase B (ARSB)
- 生物活性
- Active
- 产品特性
- AA 81-533
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Arylsulfatase B protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 80 %
- Biological Activity Comment
- Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ARSB with uPAR.
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Arylsulfatase B (ARSB) (AA 166-265) protein (GST tag)
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Arylsulfatase B (ARSB) (AA 37-533) protein (His-SUMO Tag)ARSB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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Arylsulfatase B (ARSB) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ARSB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Arylsulfatase B (ARSB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ARSB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 7.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Arylsulfatase B (ARSB)
- Abstract
- ARSB 产品
- 别名
- ASB Protein, G4S Protein, MPS6 Protein, 1110007C02Rik Protein, AI480648 Protein, As-1 Protein, As-1r Protein, As-1s Protein, As-1t Protein, As1 Protein, As1-r Protein, As1-s Protein, As1-t Protein, Asr-1 Protein, Ast-1 Protein, ARSB Protein, arylsulfatase B Protein, arylsulfatase Protein, arylsulfatase b Protein, ARSB Protein, Arsb Protein, RB348 Protein, LOC5566067 Protein, CpipJ_CPIJ011047 Protein, VDBG_03275 Protein, arsb Protein, LOC5579667 Protein
- 背景
- Alternative Names: ASB, G4S, N-acetylgalactosamine-4-sulfatase
- 分子量
- 55kDa
- UniProt
- P15848
- 途径
- Glycosaminoglycan Metabolic Process
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