CYP1B1 Protein (AA 374-516) (His tag)
-
- 抗原 See all CYP1B1 蛋白
- CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))
- 生物活性
- Active
- 产品特性
- AA 374-516
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This CYP1B1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Cytochrome P450 family 1 subfamily B member 1 (CYP1B1) is an enzyme, Which belongs to the cytochrome P450 superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1B1 with HSPb2.
- Top Product
- Discover our top product CYP1B1 蛋白
-
Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1) (AA 453-542) protein (GST tag)
CYP1B1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1) (AA 1-543) protein (Strep Tag)Crystallography grade CYP1B1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1) (AA 1-543) protein (Strep Tag)Crystallography grade CYP1B1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
-
- 应用备注
- Isoelectric Point: 7.9
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- 抗原
- CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))
- 别名
- Cytochrome P450 1B1 (CYP1B1) (CYP1B1 产品)
- 别名
- CP1B Protein, CYPIB1 Protein, GLC3A Protein, P4501B1 Protein, P4501b1 Protein, CYP1B1 Protein, CYP1B Protein, zgc:136223 Protein, cytochrome P450 family 1 subfamily B member 1 Protein, cytochrome P450, family 1, subfamily b, polypeptide 1 Protein, cytochrome P450, family 1, subfamily B, polypeptide 1 Protein, cytochrome P450 1B1 Protein, CYP1B1 Protein, Cyp1b1 Protein, cyp1b1 Protein, LOC100719054 Protein
- 背景
- Alternative Names: CP1B, GLC3A, Cytochrome P450,Family 1,Subfamily B,Polypeptide 1, Glaucoma 3,Primary Infantile
- 分子量
- 18/16kDa
- UniProt
- Q16678
- 途径
- Steroid Hormone Biosynthesis
-
