PON1 Protein (AA 35-206) (His tag)
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- 抗原 See all PON1 蛋白
- PON1 (Paraoxonase 1 (PON1))
- 生物活性
- Active
- 产品特性
- AA 35-206
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PON1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PON1 with CLU.
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- Discover our top product PON1 蛋白
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Paraoxonase 1 (PON1) (AA 1-355) protein (His tag)
PON1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Paraoxonase 1 (PON1) (AA 246-355) protein (GST tag)PON1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Paraoxonase 1 (PON1) proteinPON1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA, DB, IHC
Paraoxonase 1 (PON1) (AA 2-355) protein (His tag)Crystallography grade PON1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Paraoxonase 1 (PON1) (AA 2-355) protein (His tag)Crystallography grade PON1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 5.1
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- PON1 (Paraoxonase 1 (PON1))
- Abstract
- PON1 产品
- 别名
- esa Protein, pon Protein, pon2 Protein, MGC53915 Protein, MGC89222 Protein, PON1 Protein, ESA Protein, MVCD5 Protein, PON Protein, Pon Protein, paraoxonase 2 Protein, paraoxonase 1 Protein, pon2 Protein, PON1 Protein, Pon1 Protein
- 背景
- Alternative Names: ESA, PON, Esterase A, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, A-esterase 1, Serum aryldialkylphosphatase 1
- 分子量
- 30kDa
- UniProt
- P27169
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