ALDH1A1 Protein (AA 2-501) (His tag)
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- 抗原 See all ALDH1A1 蛋白
- ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
- 生物活性
- Active
- 产品特性
- AA 2-501
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ALDH1A1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ALDH1A1 with IL21.
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- Discover our top product ALDH1A1 蛋白
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Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 1-501) protein (GST tag)
ALDH1A1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 1-110) protein (GST tag)ALDH1A1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 2-501) protein (His tag)Crystallography grade ALDH1A1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 2-501) protein (His tag)Crystallography grade ALDH1A1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) protein (Myc-DYKDDDDK Tag)ALDH1A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 6.3
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
- Abstract
- ALDH1A1 产品
- 别名
- ALDC Protein, ALDH-E1 Protein, ALDH1 Protein, ALDH11 Protein, PUMB1 Protein, RALDH1 Protein, AL1A1 Protein, aldc Protein, aldh-e1 Protein, aldh1 Protein, aldh11 Protein, pumb1 Protein, raldh1 Protein, GGADHR Protein, ALDH1A1 Protein, ALDDH Protein, Ahd2 Protein, Aldh1 Protein, Aldh2 Protein, Ahd-2 Protein, Aldh1a2 Protein, E1 Protein, Raldh1 Protein, Aldh1a1 Protein, ALHDII Protein, RALDH 1 Protein, RalDH1 Protein, aldehyde dehydrogenase 1 family member A1 Protein, aldehyde dehydrogenase 1 family, member A1 Protein, aldehyde dehydrogenase 1 family member A1 L homeolog Protein, aldehyde dehydrogenase family 1, subfamily A1 Protein, aldehyde dehydrogenase Protein, retinal dehydrogenase 1 Protein, ALDH1A1 Protein, aldh1a1 Protein, Aldh1a1 Protein, aldh1a1.L Protein, aldH1 Protein, LOC100732581 Protein, LOC101822890 Protein
- 背景
- Alternative Names: ALDH11, ALDH1, ALDC, PUMB1, RALDH1, Retinaldehyde Dehydrogenase 1, Aldehyde dehydrogenase, cytosolic, Retinal dehydrogenase 1
- 分子量
- 58kDa
- UniProt
- P00352
- 途径
- Dopaminergic Neurogenesis
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