CYP1A1 Protein (AA 251-521) (His tag,GST tag)
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- 抗原 See all CYP1A1 蛋白
- CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
- 生物活性
- Active
- 产品特性
- AA 251-521
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CYP1A1 protein is labelled with His tag,GST tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 95 %
- Biological Activity Comment
- Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamily of enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4 (HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CYP1A1 and recombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPA4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CYP1A1 and HSPA4 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1A1 with HSPA4
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- Discover our top product CYP1A1 蛋白
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Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) (AA 1-512) protein (GST tag)
CYP1A1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) (AA 1-512) protein (Strep Tag)Crystallography grade CYP1A1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) (AA 1-524) protein (Strep Tag)Crystallography grade CYP1A1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) proteinCYP1A1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) protein (Myc-DYKDDDDK Tag)CYP1A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 6.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
- 别名
- Cytochrome P450 1A1 (CYP1A1) (CYP1A1 产品)
- 别名
- AHH Protein, AHRR Protein, CP11 Protein, CYP1 Protein, P1-450 Protein, P450-C Protein, P450DX Protein, Cyp1a2 Protein, P450-1 Protein, cyp1a1 Protein, CYP1A1 Protein, CYPIA1 Protein, Cyp45c Protein, Cypc45c Protein, P-450MC Protein, wu:fb63b04 Protein, zfCYP1A Protein, zgc:109747 Protein, ahh Protein, ahrr Protein, cp11 Protein, cyp1 Protein, cyp1a Protein, p1-450 Protein, p450-c Protein, p450dx Protein, cytochrome P450 family 1 subfamily A member 1 Protein, cytochrome P450 1A1 Protein, cytochrome P450, family 1, subfamily a, polypeptide 1 Protein, cytochrome P450, family 1, subfamily A, polypeptide 1 Protein, cytochrome P450 family 1 subfamily D polypeptide 1 Protein, cytochrome P4501A1 Protein, polycyclic hydrocarbon-inducible cytochrome P450c Protein, cytochrome P450, family 1, subfamily A Protein, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 Protein, cytochrome P450 family 1 subfamily A member 1 L homeolog Protein, CYP1A1 Protein, CpipJ_CPIJ010542 Protein, Cyp1a1 Protein, cyp1d1 Protein, LOC100328613 Protein, cyp1a Protein, LOC102129994 Protein, cyp1a1.L Protein
- 背景
- Alternative Names: CYP1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
- 分子量
- 62kDa
- UniProt
- P00185
- 途径
- Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha
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