FGF13 Protein (AA 1-192) (His tag)
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- 抗原 See all FGF13 蛋白
- FGF13 (Fibroblast Growth Factor 13 (FGF13))
- 生物活性
- Active
- 产品特性
- AA 1-192
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This FGF13 protein is labelled with His tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- FGF13 (Fibroblast growth factor 13) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF13 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF13. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF13 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF13 for 48h. The result was shown in Figure 2. It was obvious that FGF13 significantly increased cell viability of 3T3 cells.
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- Discover our top product FGF13 蛋白
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Fibroblast Growth Factor 13 (FGF13) (AA 1-245) protein (GST tag)
FGF13 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 13 (FGF13) (AA 1-245) protein (His tag)FGF13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 13 (FGF13) (AA 10-245) protein (His-IF2DI Tag)FGF13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Fibroblast Growth Factor 13 (FGF13) (AA 1-245) protein (Strep Tag)Crystallography grade FGF13 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
- Isoelectric Point: 9.2
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- FGF13 (Fibroblast Growth Factor 13 (FGF13))
- Abstract
- FGF13 产品
- 别名
- FGF13 Protein, fgf2 Protein, fhf2 Protein, fgf13 Protein, FGF-13 Protein, xFGF13 Protein, FGF2 Protein, FHF-2 Protein, FHF2 Protein, Fhf2 Protein, zgc:101784 Protein, fibroblast growth factor 13 Protein, fibroblast growth factor 13 L homeolog Protein, fibroblast growth factor 13a Protein, FGF13 Protein, fgf13 Protein, fgf13.L Protein, Fgf13 Protein, fgf13a Protein
- 背景
- Alternative Names: FHF2, Fibroblast Growth Factor Homologous Factor 2
- 分子量
- 24kDa
- UniProt
- Q9ERW3
- 途径
- Regulation of Cell Size
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