GDF9 Protein (AA 320-454) (His tag)
(1 reference)-
- 抗原 See all GDF9 蛋白
- GDF9 (Growth Differentiation Factor 9 (GDF9))
- 生物活性
- Active
- 产品特性
- AA 320-454
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GDF9 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFβ) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GDF9 with S100A8.
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- Discover our top product GDF9 蛋白
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Growth Differentiation Factor 9 (GDF9) (AA 1-454) protein (GST tag)
GDF9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Differentiation Factor 9 (GDF9) (AA 320-454) protein (His-IF2DI Tag)GDF9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Growth Differentiation Factor 9 (GDF9) (AA 320-454) protein (GST tag)GDF9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- Isoelectric Point: 7.1
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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: "Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells." in: Development (Cambridge, England), Vol. 147, Issue 13, (2020) (PubMed).
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: "Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells." in: Development (Cambridge, England), Vol. 147, Issue 13, (2020) (PubMed).
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