NUCB2 Protein (AA 26-106) (His tag,GST tag)
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- 抗原 See all NUCB2 (NES1) 蛋白
- NUCB2 (NES1) (Nesfatin-1 (NES1))
- 生物活性
- Active
- 产品特性
- AA 26-106
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NUCB2 protein is labelled with His tag,GST tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, rat GADD45A shares similarities with mouse NES1 in amino acid sequence with the identity of 98%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NES1 and recombinant rat GADD45A. Briefly, NES1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL NES1 were then transferred to GADD45A-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45A was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of NES1 with GADD45A.
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Nesfatin-1 (NES1) proteinNES1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, IP, ELISA
Nesfatin-1 (NES1) proteinNES1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, IP, ELISA
Nesfatin-1 (NES1) proteinNES1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, IP, ELISA
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- 应用备注
- Isoelectric Point: 5.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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