PTH Protein (AA 32-115) (MBP tag,His tag)
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- 抗原 See all PTH 蛋白
- PTH (Parathyroid Hormone (PTH))
- 生物活性
- Active
- 产品特性
- AA 32-115
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PTH protein is labelled with MBP tag,His tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: Two N-terminal Tags, His-tag and MBP-tag
- 纯度
- > 90 %
- Biological Activity Comment
- PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.
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Parathyroid Hormone (PTH) (Active) protein
PTH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Parathyroid Hormone (PTH) (AA 32-115) protein (Fc Tag)PTH 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag)PTH 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） > 90 % CC, AcA Active
Parathyroid Hormone (PTH) (AA 32-115) protein (His-IF2DI Tag)PTH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Parathyroid Hormone (PTH) (AA 32-115) protein (His tag)PTH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Parathyroid Hormone (PTH) (AA 1-115) protein (GST tag)PTH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Parathyroid Hormone (PTH) (AA 32-115) protein (GST tag)PTH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Isoelectric Point: 5.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- PTH (Parathyroid Hormone (PTH))
- Abstract
- PTH 产品
- 别名
- PTH1 Protein, Pthp Protein, PTH-(1-84) Protein, Pth1 Protein, Pthr1 Protein, PTH Protein, parathyroid hormone Protein, parathyroid hormone S homeolog Protein, PTH Protein, Pth Protein, pth.S Protein
- 物质类
- Hormone
- 背景
- Alternative Names: iPTH, Intact Parathyroid Hormone, Parathormone, Parathyrin
- 分子量
- 59kDa
- UniProt
- P04089
- 途径
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
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