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- 抗原 See all LCAT 蛋白
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- 生物活性
- Active
- 产品特性
- AA 41-210
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This LCAT protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of LCAT with APOA1.
- Top Product
- Discover our top product LCAT 蛋白
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Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-440) protein (His tag)
LCAT 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)LCAT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 1-440) protein (GST tag)LCAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 98-197) protein (GST tag)LCAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-440) protein (His tag)Crystallography grade LCAT 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)Crystallography grade LCAT 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Isoelectric Point: 6.3
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- 别名
- Lecithin Cholesterol Acyltransferase (LCAT) (LCAT 产品)
- 别名
- AI046659 Protein, D8Wsu61e Protein, MGC82035 Protein, lcat Protein, MGC88964 Protein, LCAT Protein, lecithin-cholesterol acyltransferase Protein, lecithin cholesterol acyltransferase Protein, lecithin-cholesterol acyltransferase L homeolog Protein, solute carrier family 12 member 4 Protein, fragile site, aphidicolin type, common, fra(13)(q13.2) Protein, LCAT Protein, Lcat Protein, lcat.L Protein, lcat Protein, SLC12A4 Protein, FRA13A Protein
- 背景
- Alternative Names: phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase
- 分子量
- 22kDa
- UniProt
- P16301
- 途径
- Lipid Metabolism
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