IL10RB Protein (AA 87-243) (His tag)
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- 抗原 See all IL10RB 蛋白
- IL10RB (Interleukin 10 Receptor, beta (IL10RB))
- 生物活性
- Active
- 产品特性
- AA 87-243
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL10RB protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of IL10Rb with IL10.
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Interleukin 10 Receptor, beta (IL10RB) (AA 20-119) protein (GST tag)
IL10RB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 10 Receptor, beta (IL10RB) (Active) protein (His tag)IL10RB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
Interleukin 10 Receptor, beta (IL10RB) protein (Fc Tag)IL10RB 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 10 Receptor, beta (IL10RB) protein (Fc Tag)IL10RB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE
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- 应用备注
- Isoelectric Point: 5
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL10RB (Interleukin 10 Receptor, beta (IL10RB))
- 别名
- Interleukin 10 Receptor Beta (IL10Rb) (IL10RB 产品)
- 别名
- IL10R2 Protein, IL10RB Protein, il10r2 Protein, CDW210B Protein, CRF2-4 Protein, CRFB4 Protein, D21S58 Protein, D21S66 Protein, IL-10R2 Protein, 6620401D04Rik Protein, AI528744 Protein, Crfb4 Protein, D16H21S58 Protein, D21S58h Protein, Il10r2 Protein, RGD1560373 Protein, interleukin 10 receptor subunit beta Protein, interleukin 10 receptor subunit beta S homeolog Protein, interleukin-10 receptor subunit beta Protein, interleukin 10 receptor, beta Protein, IL10RB Protein, il10rb.S Protein, LOC473966 Protein, LOC478404 Protein, il10rb Protein, Il10rb Protein
- 背景
- Alternative Names: CDW210b, CD210-B, IL10R-B, IL10-RB, CRF2-4, CRFB4, IL-10R2, Interleukin-10 receptor subunit 2
- 分子量
- 18kDa
- UniProt
- Q08334
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