GZMM Protein (AA 26-257) (His tag)
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- 抗原 See all GZMM 蛋白
- GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))
- 生物活性
- Active
- 产品特性
- AA 26-257
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GZMM protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2x107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin. Normal Heated 0 4 4 GZMM (μM) Ezrin Actin Figure 1. GZMM cleaves Ezrin
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Granzyme M (Lymphocyte Met-Ase 1) (GZMM) (AA 85-193) protein (GST tag)
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Granzyme M (Lymphocyte Met-Ase 1) (GZMM) (AA 1-257) protein (GST tag)GZMM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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Granzyme M (Lymphocyte Met-Ase 1) (GZMM) proteinGZMM 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Granzyme M (Lymphocyte Met-Ase 1) (GZMM) proteinGZMM 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- Isoelectric Point: 10.3
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))
- 别名
- Granzyme M (GZMM) (GZMM 产品)
- 别名
- LMET1 Protein, MET1 Protein, Lmet1 Protein, MMET-1 Protein, granzyme M Protein, granzyme M (lymphocyte met-ase 1) Protein, LOC100136381 Protein, GZMM Protein, Gzmm Protein
- 背景
- Alternative Names: GZM-M, LMET1, MET1, Hu-Met-1, Met-ase, Lymphocyte Met Ase 1, Met-1 serine protease, Natural killer cell granular protease
- 分子量
- 26kDa
- UniProt
- P51124
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