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- 抗原 See all IDO 蛋白
- IDO (Indoleamine 2,3-Dioxygenase (IDO))
- 生物活性
- Active
- 产品特性
- AA 2-403
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IDO protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. Briefly, a mixture of 800μM L-tryptophan, 9000 units/mL catalase, and 40μM Methyene Blue with the addition of 80mM ascorbic acid reaction buffer was incubated with different concentrations of IDO, and the absorbance was read in 321nm every 1.5h. The result indicated that recombinant human IDO oxidize L-tryptophan Enzyme activity of recombinant human IDO.
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Indoleamine 2,3-Dioxygenase (IDO) protein
IDO 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
Indoleamine 2,3-Dioxygenase (IDO) proteinIDO 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- Isoelectric Point: 6.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % skl, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IDO (Indoleamine 2,3-Dioxygenase (IDO))
- 别名
- Indoleamine-2,3-Dioxygenase (IDO) (IDO 产品)
- 别名
- IDO Protein, IDO-1 Protein, INDO Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, IDO1 Protein, Ido1 Protein
- 背景
- Alternative Names: CD107B, INDO, Indoleamine-Pyrrole 2,3 Dioxygenase
- 分子量
- 43kDa
- UniProt
- P14902
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