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IL17RD Protein (AA 157-299) (His tag)

IL17RD 宿主: 人 宿主: 大肠杆菌(E. Coli) > 90 % AcA, CC Active
产品编号 ABIN6239809
发货至: 中国
  • 抗原 See all IL17RD 蛋白
    IL17RD (Interleukin 17 Receptor D (IL17RD))
    生物活性
    Active
    产品特性
    AA 157-299
    宿主
    • 7
    • 4
    • 1
    • 1
    • 1
    资源
    • 6
    • 3
    • 2
    • 2
    • 1
    大肠杆菌(E. Coli)
    标记
    This IL17RD protein is labelled with His tag.
    应用范围
    Activity Assay (AcA), Cell Culture (CC)
    产品特性
    Tag location: N-terminal His Tag
    纯度
    > 90 %
    Biological Activity Comment
    IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in Figure 1 and Figure 2 respectively and this effect was in a dose dependent manner The binding activity of IL17RD with IL17RA. Figure 2. The binding activity of IL17RD with IL17.
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  • 应用备注
    Isoelectric Point: 8.9
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    储存液
    Dithiothreitol (DTT), Other preservative, ProClin
    注意事项
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • 抗原
    IL17RD (Interleukin 17 Receptor D (IL17RD))
    Abstract
    IL17RD 产品
    别名
    IL17RD Protein, sef Protein, il-17rd Protein, il17rlm Protein, HH18 Protein, IL-17RD Protein, IL17RLM Protein, SEF Protein, 2810004A10Rik Protein, AI428510 Protein, Sef Protein, Sef-S Protein, RGD1565237 Protein, cb208 Protein, id:ibd2875 Protein, id:ibd5059 Protein, zgc:136328 Protein, interleukin 17 receptor D Protein, IL17RD Protein, il17rd Protein, Il17rd Protein
    背景
    Alternative Names: SEF, IL17RLM, IL-17RD, IL17R-D, IL17Rhom, Interleukin-17 receptor-like protein
    分子量
    20kDa
    UniProt
    Q8NFM7
    途径
    Toll-Like Receptors Cascades
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