S100A6 Protein (AA 1-90) (His tag)
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- 抗原 See all S100A6 蛋白
- S100A6 (S100 Calcium Binding Protein A6 (S100A6))
- 生物活性
- Active
- 产品特性
- AA 1-90
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This S100A6 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of S100A6 with RAGE.
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S100 Calcium Binding Protein A6 (S100A6) (AA 1-90) protein (His-IF2DI Tag)
S100A6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
S100 Calcium Binding Protein A6 (S100A6) (AA 1-90) protein (GST tag)S100A6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
S100 Calcium Binding Protein A6 (S100A6) (AA 18-90) protein (GST tag)S100A6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Isoelectric Point: 5.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- S100A6 (S100 Calcium Binding Protein A6 (S100A6))
- Abstract
- S100A6 产品
- 别名
- S100A6 Protein, 2A9 Protein, 5B10 Protein, CABP Protein, CACY Protein, PRA Protein, CALCYCLIN Protein, Cacy Protein, S100 calcium binding protein A6 Protein, uncharacterized LOC618256 Protein, S100 calcium binding protein A6 (calcyclin) Protein, S100A6 Protein, S100a6 Protein, LOC618256 Protein
- 背景
- Alternative Names: S100-A6, 2A9, 5B10, CABP, CACY, PRA, MLN 4, Calcyclin, Growth factor-inducible protein 2A9, Prolactin receptor-associated protein
- 分子量
- 16kDa
- UniProt
- P06703
- 途径
- S100 Proteins
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