CIRBP Protein (AA 1-172) (His tag)
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- 抗原 See all CIRBP 蛋白
- CIRBP (Cold Inducible RNA Binding Protein (CIRBP))
- 生物活性
- Active
- 产品特性
- AA 1-172
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CIRBP protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CIRBP with ATXN1.
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- Discover our top product CIRBP 蛋白
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Cold Inducible RNA Binding Protein (CIRBP) (AA 1-172) protein (GST tag)
CIRBP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cold Inducible RNA Binding Protein (CIRBP) (AA 1-172) protein (His-IF2DI Tag)CIRBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cold Inducible RNA Binding Protein (CIRBP) (AA 1-90) protein (GST tag)CIRBP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cold Inducible RNA Binding Protein (CIRBP) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CIRBP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 9.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- CIRBP (Cold Inducible RNA Binding Protein (CIRBP))
- Abstract
- CIRBP 产品
- 别名
- CIRP Protein, Cirp Protein, R74941 Protein, Xcirp Protein, cirbp Protein, cirbp-a Protein, cirbp2 Protein, cirp Protein, cirp1 Protein, cirp2 Protein, xcirp2 Protein, XCIRP Protein, XCIRP-1 Protein, cirbp-b Protein, cirp-1 Protein, APBP-1 Protein, zgc:136559 Protein, CIRBP Protein, A18 hnRNP Protein, cold inducible RNA binding protein Protein, cold-inducible RNA-binding protein Protein, cold inducible RNA binding protein S homeolog Protein, cold inducible RNA binding protein L homeolog Protein, cold inducible RNA binding protein b Protein, CIRBP Protein, EDI_107320 Protein, Cirbp Protein, cirbp.S Protein, cirbp.L Protein, cirbp Protein, cirbpb Protein
- 背景
- Alternative Names: CIRP, A18HNRNP, Glycine-Rich RNA Binding Protein
- 分子量
- 22/27kDa
- UniProt
- Q14011
- 途径
- Ribonucleoprotein Complex Subunit Organization
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